Common technologies employed in upstream cell harvesting include centrifugation, tangential-flow filtration, and depth filtration, often times coupled together in larger scale operations. This new concept in single-use filtration separates solids from suspension using dead-end filtration to deposit a cake. Flat filter elements are packed close together inside a multi-layered plastic bag enclosure to provide room for solid depositions up to 8 mm thick and to maximize surface area. The plastic bag is designed with multiple layers to be able to withstand chemical environments, undergo high pressures, and reduce gas permeation. The plastic filter bag is installed in a pressure vessel to allow the bag to be compressed via gas to dewater the cake. This enables filtration of the liquid heel so that there is virtually no hold-up left in the filter chamber. The filter elements are constructed in HDPE with an average pore size between 0.8 – 1 μm. These elements are flexible and conform to the shape of the bag when external pressure is applied. These elements are also back-washable via a back- flush tank which uses pressurized filtrate to discharge the deposited solids from the filter media and let them settle to the bottom of the filter bag. This enables the filter to perform multiple filtration cycles prior to disposal since solids can accumulate from cycle to cycle until the bag is full. Once full, the bag can be compressed to recover the hold-up volume, and the bag containing the solid waste can be removed and disposed of safely. In-situ filtration, cake washing, dewatering, and discharging provide a simple operation that reduces equipment costs and increases overall throughput.